刘彦梅，博士，研究员，博士生导师。本科与硕士毕业于北京大学医学部临床医学专业，在新加坡国立大学获得生物学博士学位，曾于德国马克斯普朗克研究所/德累斯顿工业大学医学院做博士后，北京大学分子医学研究所任副研究员，2018年9月以青年拔尖人才引进方式加盟华南师范大学脑科学与康复医学研究院。长期致力于胰岛细胞发育与再生机制的研究。近年来研究兴趣扩展为改造和应用基因编辑技术构建单基因脑疾病的斑马鱼模型，并利用斑马鱼脑疾病模型进行病理机制研究以及药物筛选。其一系列研究成果以第一作者或(共)通讯作者发表在《Blood》、《Nature Communications》、《Developmental Cell》、《Elife》、《Diabetes Obesity and Metabolism》、《Journal of Cell Science》等杂志。
代表论著：
第一及（共）通讯作者文章：
(1) Zhang Y, Liu Y, Qin W, Zheng S, Xiao J, Xia X, Yuan X, Zeng J, Shi Y, Zhang Y, Ma H, Varshney GK*, Fei JF*, Liu Y*. Cytosine base editors with increased PAM and deaminase motif flexibility for gene editing in zebrafish. Nat Commun. 2024 Nov 4;15(1):9526. doi: 10.1038/s41467-024-53735-y.
(2) Qin W, Liang F, Lin SJ, Petree C, Huang K, Zhang Y, Li L, Varshney P, Mourrain P, Liu Y*, Varshney GK*. ABE-ultramax for high-efficiency biallelic adenine base editing in zebrafish. Nat Commun. 2024 Jul 4;15(1):5613. doi: 10.1038/s41467-024-49943-1.
(3) Cui X, Li X, Peng C, Qiu Y, Shi Y, Liu Y*, Fei JF*. Beyond External Light: On-Spot Light Generation or Light Delivery for Highly Penetrated Photodynamic Therapy. ACS Nano. 2023 Nov 14;17(21):20776-20803.
(4) Fu S, Peng C, Zeng YY, Qiu Y, Liu Y*, Fei JF*. Establishing an Efficient Electroporation-Based Method to Manipulate Target Gene Expression in the Axolotl Brain. Cell Transplant. 2023 Jan-Dec;32:9636897231200059.
(5) Zheng S, Liang F, Zhang Y, Fei JF, Qin W*, Liu Y*. Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e. J Vis Exp. 2023 Feb 17;(192).
(6) Resolving vertebrate brain evolution through salamander brain development and regeneration. Pan XY, Zeng YY, Liu YM*, Fei JF*. Zool Res. 2023 Jan 18;44(1):219-222.
(7) Wang L, Zeng YY, Liu Y*, Fei JF*. Applying a Knock-In Strategy to Create Reporter-Tagged Knockout Alleles in Axolotls. Methods Mol Biol. 2023; 2562:351-368.
(8) 彭程, 刘彦梅*, 费继锋* 神经环路再生研究进展. 中国细胞生物学学报 Chinese Journal of Cell Biology 2022, 44(8): 1529–1541
(9) Liang F, Zhang Y, Li L, Yang Y, Fei JF, Liu Y*, Qin W*. SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish. Nat Commun. 2022 Jun 14;13(1):3421.
(10) Zhang Y, Han C, Zhu W, Yang G, Peng X, Mehta S, Zhang J, Chen L*, Liu Y*. Glucagon Potentiates Insulin Secretion Via β-Cell GCGR at Physiological Concentrations of Glucose. Cells 2021, 10, 2495.
(11) Zhao J, Liang S, Zhao Y, Zong W, Tran E, Chen L, Liu Y. In vivo Imaging of Calcium Activities from Pancreatic β-cells in Zebrafish Embryos Using Spinning-disc Confocal and Two-photon Light-sheet Microscopy. Bio Protoc. 2021 Dec 5;11(23):e4245.
(12) Liu Y, Lou W, Fei JF. The engine initiating tissue regeneration: does a common mechanism exist during evolution? Cell Regen. 2021 Apr 5;10(1):12.
(13) Kang F, Zhou M, Huang X, Fan J, Wei L, Boulanger J, Liu Z, Salamero J, Liu Y*, Chen L*. E-syt1 Re-arranges STIM1 Clusters to Stabilize Ring-shaped ER-PM Contact Sites and Accelerate Ca2+ Store Replenishment. Sci Rep. 2019 Mar 8;9(1):3975.
(14) Jia Zhao; Weijian Zong; Yiwen Zhao; Dongzhou Gou; Shenghui Liang; Jiayu Shen; Yi Wu; Xuan Zheng; Runlong Wu; Xu Wang; Fuzeng Niu; Aimin Wang; Yunfeng Zhang; Jing Wei Xiong; Liangyi Chen*; Yanmei Liu*; In vivo imaging of β-cell function reveals glucose-mediated heterogeneity of β-cel l functional development, eLife, 2019, 8: 0-e41540
(15) Wang Y, Han C, Zhu W, Wu Z, Liu Y*, Chen L*. An optical method to evaluate both mass and functional competence of pancreatic alpha- and beta-cells. Journal of Cell Science. 2016;129(12):2462-71. doi: 10.1242/jcs.184523. (Cover story)
(16) Tianyi Yuan; Lin Liu; Yongdeng Zhang; Lisi Wei; Shiqun Zhao; Xiaolu Zheng; Xiaoshuai Huan g; Jerome Boulanger; Charles Gueudry; Jingze Lu; Lihan Xie; Wen Du; Weijian Zong; Lu Yang; Jean S alamero; Yanmei Liu*; Liangyi Chen* ; Diacylglycerol Guides the Hopping of Clathrin-Coated Pits alo ng Microtubules for Exo-Endocytosis Coupling, Developmental Cell, 2015, 35(1): 120-130
(17) 刘彦梅*, 郑晓璐，袁天一，陈良怡* 可兴奋细胞胞吐胞吞的耦联机制。中国细胞生物学学报 2015; (12):1606-15.
(18) Liu Y, Suckale J, Masjkur J, Magro MG, Steffen A, Anastassiadis K, Solimena M. Tamoxifen-independent recombination in the RIP-CreER mouse. PLoS One. 2010 Oct 22;5(10):e13533.
(19) Liu Y, Mziaut H, Ivanova A, Solimena M.beta-Cells at the crossroads: choosing between insulin granule production and proliferation. Diabetes Obes Metab. 2009 Nov;11 Suppl 4:54-64.
(20) Yanmei Liu; Linsen Du; Motomi Osato; Eng Hui Teo; Feng Qian; Hao Jin; Fenghua Zhen; Jin X u; Lin Guo; Honghui Huang; Jun Chen; Robert Geisler; Yun-Jin Jiang,; Jinrong Peng; Zilong Wen ; T he zebrafish udu gene encodes a novel nuclear factor and is essential for primitive erythroid cel l development., Blood, 2007, 110(1): 99-106
合作作者文章：
(1) Zhao J, Liang S, Cen HH, Li Y, Baker RK, Ruprai B, Gao G, Zhang C, Ren H, Tang C, Chen L, Liu Y, Lynn FC, Johnson JD, Kieffer TJ. PDX1+ cell budding morphogenesis in a stem cell-derived islet spheroid system. Nat Commun. 2024 Jul 13;15(1):5894.
(2) Zhang XS, Wei L, Zhang W, Zhang FX, Li L, Li L, Wen Y, Zhang JH, Liu S, Yuan D, Liu Y, Ren C, Li S.. ERK-activated CK-2 triggers blastema formation during appendage regeneration. Sci Adv. 2024 Mar 22;10(12):eadk8331.
(3) Hu Y, Pan X, Shi Y, Qiu Y, Wang L, Murawala P, Liu Y, Xing W, Tanaka EM, Fei JF. Muscles are barely required for the patterning and cell dynamics in axolotl limb regeneration. Front Genet. 2022 Oct 10;13:1036641.
(4) Zhao W, Zhao S, Li L, Huang X, Xing S, Zhang Y, Qiu G, Han Z, Shang Y, Sun DE, Shan C, Wu R, Gu L, Zhang S, Chen R, Xiao J, Mo Y, Wang J, Ji W, Chen X, Ding B, Liu Y, Mao H, Song BL, Tan J, Liu J, Li H, Chen L. Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy. Nat Biotechnol. 2021 Nov 15.
(5) Yang G, Li L, Liu Y, Liang K, Wei L, Chen L. Hyperglycemia-Induced Dysregulated Fusion Intermediates in Insulin-Secreting Cells Visualized by Super-Resolution Microscopy. Front Cell Dev Biol. 2021 Apr 15;9:650167.
(6) Dong D, Huang X, Li L, Mao H, Mo Y, Zhang G, Zhang Z, Shen J, Liu W, Wu Z, Liu G, Liu Y, Yang H, Gong Q, Shi K, Chen L. Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome. Light Sci Appl. 2020 Jan 28;9:11.
(7) Zhang Y, Wang J, Xing S, Li L, Zhao S, Zhu W, Liang K, Liu Y, Chen L. Mitochondria determine the sequential propagation of the calcium macrodomains revealed by the super-resolution calcium lantern imaging. Sci China Life Sci. 2020 Oct;63(10):1543-1551.
(8) Huang X, Fan J, Li L, Liu H, Wu R, Wu Y, Wei L, Mao H, Lal A, Xi P, Tang L, Zhang Y, Liu Y, Tan S*, Chen L*. Ultrasensitive Hessian structured illumination microscopy enables ultrafast and long-term super-resolution imaging. Nature Biotechnology. 2018; 36(5):451-459. doi: 10.1038/nbt.4115.
(9) Ivanova A, Kalaidzidis Y, Dirkx R, Sarov M, Gerlach M, Schroth-Diez B, Müller A, Liu Y, Andree C, Mulligan B, Münster C, Kurth T, Bickle M, Speier S, Anastassiadis K, Solimena M. Age-dependent labeling and imaging of insulin secretory granules. Diabetes. 2013 Nov;62(11):3687-96.
(10) Du L, Xu J, Li X, Ma N, Liu Y, Peng J, Osato M, Zhang W, Wen Z. Rumba and Haus3 are essential factors for the maintenance of hematopoietic stem/progenitor cells during zebrafish hematopoiesis. Development. 2011 Feb;138(4):619-29.
授权发明专利:
(1) 刘彦梅;费继锋;阳磊;李响；一种条件性诱导神经干细胞增殖的墨西哥钝口螈动物模型的构建方法，2023-12-22，中国，ZL 202210719774.4 (发明专利)
(2) 刘彦梅;付苏雷;费继锋;曾雁云;胡燕;王鹍；一种神经干细胞特异性消融动物模型的构建方法及其应用，2024-04-30，中国，ZL 202111289115.3 （发明专利）
(3) 刘彦梅;张煜;郑少辉;费继锋；一种斑马鱼胞嘧啶碱基编辑器及其构建方法与应用，2024-05-24，中国，：ZL 2023 1 0872424.6 （发明专利）
(4) 刘彦梅;赵佳; 陈良怡; 一种在体筛选促胰岛β细胞功能药物的方法, 2021-09-14, 中国, ZL 201710010182.4 (发明专利)
承担科研项目情况：
1. 国家自然科学基金面上项目（32070819）：活体实时成像研究胰岛三种内分泌细胞协同发育和获得功能的机制, 2021/01-2024/12，主持
2. 深圳华大生命科学研究院开放基金项目（BGIRSZ20210002）：结合时空组学技术研究蝾螈胰岛β细胞再生机制，2022/01-2023/12，主持
3. 国家重点研发计划国际合作重点专项（2019YFE0106700）：应用墨西哥钝口螈鉴定直接参与组织再生的细胞类型及其启动再生的关键因子，2020/07-2022/06，骨干
4. 广东省科技厅农村科技特派员项目（KTP20200110）：水生动物的标准化养殖，2020/07-2022/06，骨干
5. 国家自然科学基金重大研究计划培育项目(91854112)： 应用海森结构光超高分辨率显微镜研究靶向溶酶体的线粒体衍生囊泡的发生机制，2019/01-2021/12，主持
6. 国家自然科学基金重大研究计划重点项目（91750203）：基于光场调控的光学成像新技术与应用研究，2018/01-2021/12，骨干
7. 北京市自然科学基金面上项目（7152079）：瑞特综合征致病因子MeCP2调控胰岛细胞重新编程的机制及其在糖尿病发病中的作用，2015/01-2017/12，主持
8. 国家自然科学基金青年项目（31301186）：利用斑马鱼在体荧光成像研究胰岛beta 细胞的分泌和成熟机制，2014/01-2016/12，主持
欢迎志同道合的硕士生、博士生、博士后及青年英才的加盟,让我们在科研道路上一起携手,不忘初心,砥砺前行! 联系邮箱：yanmeiliu@m.scnu.edu.cn